Shoot culture 9most widely used method for commercial micropropagation 9relatively high genetic stability in the plants produced shoot culture advantages 9reliable rates and consistency of shoot multiplication 93 8 fold multiplication rate per month 9preexisting meristems are least susceptible to genetic changes micropropagation stages stage 0. Pierre leguminaceae, subfamily papilionoideae is a medium sized tree commonly called as karanja that generally attains a height of about 1020 m and a trunk diameter of more than 50 cm pongamia. Therefore, micropropagation of bud material was attempted as a method of introduction of woody plants to the botanical garden. The purpose of stagei is to initiate axenic culture. Evaluation of antibacterial activity of pongamia pinnata l. Generally, disease free mother explant is selected for the micropropagation to reduce contamination of cultures. Pongamia pinnata is a legume plant which has great potential to be used as a. Effects of pongamia pinnata on lipid peroxidation products and antioxidants in hyperammonemic rats with reference to circadian variations were evaluated by essa and subramanian 29. Recent studies2 4 revealed that most chlorophyllous plants in vitro have the ability to grow photoautotrophically, and that the low co 2 concentration in the airtight. Jain 1, shashi alok 1, deepak kailasiya 1, vinod kr. Axillary buds, biodiesel plant, leaf, micropropagation, pongamia, plant growth regulators, plant tissue culture. Micropropagation is the practice of rapidly multiplying stock plant material to produce many progeny plants, using modern plant tissue culture methods micropropagation is used to multiply plants such as those that have been genetically modified or bred through conventional plant breeding methods. Micropropagation of ornamental perennials university of. Micropropagation is a combination of the arts and sciences of plant multiplication in vitroand plant acclimitization.
A protocol for in wk propagation of this plant was standardized using seedling explants. A piece of plant tissue called an explant is a cut from the plant, b disinfested removal of surface contaminants, and c. Principles of tissue cult ure and micropropagation. This is particularly valuable in the case of difficultto. Micropropagation differs from all other conventional propagation methods in that aseptic conditions are essential to achieve success. Zg gzr kl ghlbiz journal of central european agriculture. Analysis of genetic diversity in pongamiapongamia pinnata. Pdf pongamia pinnata, a legume tree, has many traditional uses and is a potential biodiesel plant.
Harnessing potential of selected underutilized bio energy. Multiple shoot bud induction and plant regeneration studies of. Millettia pinnata is a species of tree in the pea family, fabaceae, native to eastern and tropical asia, australia and pacific islands. Pdf pongamia pinnata is an oilproducing tree species with multiple uses and considerable potential as a bioenergy crop. S department of agriculture was given seeds from sri lanka. The process of micropropagation can be divided into four stages. Pongamia millettia pinnata, formerly known as pongamia pinnata, is a treeshrub. Micropropagation of orchids micropropagation is the process used to replicate plants such as orchids, the word micropropagation means simply propagation in miniature. Plant propagation by tissue culture is known as micropropagation. Pdf molecular markerbased characterization in candidate plus. Micropropagation and regeneration of herbaceous perennials native to the eastern united states. Uses, benefits, cures, side effects, nutrients in pongamia pinnata. Gipb case study pongamia pinnata godbole india draft final.
Micropropagation of pongamia pinnata through enhanced. Pongamia pinnata herb uses, benefits, cures, side effects. Preparation of biodiesel from crude oil of pongamia. A rapid, simple and specific reversedphase hplc method has been developed for analysis of karanjin in pongamia pinnata linn. These endogenously produced auxins can combine with cytokinins present. Pongamia pinnata, floral phenology, pod maturation, fruit colours, seed and seedling quality characters. Micropropagation of mature pongamia pinnata pierre.
Of the various culture media that have been assayed for micropropagation of. Lucknow, who introduced us to plant tissue culture and to the intricacies of the. Our pdf merger allows you to quickly combine multiple pdf files into one single pdf document, in just a few clicks. The state of the art a practical guide to woody plant micropropagation john w einset the spinoff from basic research on the physiology of plants, plant micropropagation is a simple, straightforwardand commercially profitable technique although its commercial use as a method for multiplying plants is still fairly new, tissue culture propagation micropropagation has.
Literature survey of pongamia pinnata revealed its potential being like hepatoprotective and antioxidant 30. Pongamia pinnata is a legume plant which has great potential to be used as a biofuel feedstock. Employing plant tissue culture techniques for micropropagation and further. Pdf inter and intrapopulation variability of pongamia pinnata.
In vitro micropropagation of biofuel feedstock pongam pongamia. Micropropagation is labour intensive involving manual handling at 3 or 4 stages. International journal of pharmtech research coden usa. With orchids it is the only way to reproduce the plants as. It is done under sterile conditions in a laboratory using plant seed or tissue. A complete protocol is presented for the first time for the micropropagation of pongamia pinnata, a biofuel tree, using cotyledonary nodes derived from axenic seedlings.
Learn how to combine pdfs and reorder, rotate, and delete pages. Incorporation of ba, kn, or z was ineffective in enhancing sprouting frequency or. Development and validation of an hplc method for karanjin. Curcuma longa is an indian spice derived from the rhizomes of the plant and has a long history of use in ayurvedic medicine as a treatment for inflammatory conditions. Details of the excursion, seed material collected and its horticultural consequences are to be found in koponen and koponen 1994, 1995, 1997 and koponen 1998. Advantages of micropropagation requires relatively small growing area gives propagules such as minitubersmicrocorms for plant multiplication throughout the year. Introduction to micropropagation cornell university. One such genus is that of trilliums, and while i do not intend to work on trilliums exclusively, i do intend to concentrate on this group. Micropropagation in horticulture, by s gunn the plantsman new series vol. How pongamia pinnata is effective for various diseases is listed in repertory format. General physical properties of pongamia pinnata oil blends b10, b20 and diesel parameters pongamia pinnata diesel b20b10 ph 3.
Pdf micropropagation of mature pongamia pinnata pierre. Hplc analysis was performed on a c 18 column using an 85. It grows about 1520 meters in height with a large canopy which spreads equally wide. The potato micropropagation armstatehydromet and ministry of agriculture yerevan, march 2012 rolot jeanlouis. It is an update to an article hyndman, 1987 originally published in aroideana, the journal of the international aroid society. Chapter2 micropropagation and large scale culture of c. Names of pongamia pinnata in various languages of the world are also given. The rhs libraries can, for a fee, provide photocopies, by post if necessary. Micropropagation is an advanced vegetative propagation technology for producing a large number of genetically superior and pathogenfree transplants in a limited time and space. Development of micropropagation protocols would allow commercial tissue culture companies to mass propagate and make available to the nursery trade elite lines of selected ornamental, herbaceous perennials. How to merge pdfs and combine pdf files adobe acrobat dc. It is found mainly in the native western ghats in india, northern australia, fiji and in some regions of eastern asia.
Department of pharmacognosy 1, small and medium pharmaceutical industry centre 2, institute of. In protocols for micropropagation of selected economicallyimportant horticultural plants, well recognised researchers in the field compile stepwise protocols for rapid plant multiplication of economicallyimportant horticultural species. Enhancing rate of multiplication shoot multiplication short cycle 26 weeks small size propagules storage on large scale, easily transported. This web page is only meant to be an introduction to the tissue culture propagation technique known as micropropagation for some of the plants composing the family araceae l. Nekl, assistant professor of biology cofaculty proposers. Research article physico chemical analysis of pongamia. Pierrea native indian biodiesel tree from cotyledonary node. The micropropagation of chestnut via axillary shoots involves four stages. It is the truetotype propagation of a genotype that comprises many stepsstock plant care, explant selection and sterilization, media manipulation to obtain proliferation, rooting, acclimation, and growing on of liners and is usually. Micropropagation micropropagation is the production of whole plants from small sections of plant such as a shoot tip, node, meristem, embryo, or even a seed plant tissue culture is basically the same thing, except that it implies the use of callus tissue generated from plant cells cultured invitro. Pongamia pinnata has recently been recognized as a viable source of seedoil for production of biofuel to meet the demand of the burgeoning. Native to tropical and subtropical areas, the pongam tree, or pongamia pinnata, was first introduced to hawaii in the 1860s. Yeole2 1sanjivani college of pharmaceutical education and research, kopergaon, india.
It is often known by the synonym pongamia pinnata as it was moved to the genus millettia only recently. A free powerpoint ppt presentation displayed as a flash slide show on id. Pongamia pinnata oil with conventional diesel with b10 and b20 proportions are furnished in the table3. Protocols for micropropagation of selected economically. Before initiation of micropropagation, selection of suitable mother plant is crucial in the whole exercise of propagation. It is also used to provide a sufficient number of plantlets for planting from a stock plant. Easily combine multiple files into one pdf document. After tranesterification of crude oil shows excellent properties like calorific value, iodine number, cetane number and acid value etc. According to murashige of california university, micropropagation is distinctly divided into five stages. Practical guide woody micropropagation arnold arboretum. The method was validated for accuracy, precision, linearity. Pongamia pinnata pierre is a tree legume, having potential in production of raw material for biodiesel. Micropropagation is the truetotype propagation of a selected genotype using in vitro culture techniques.
Pinnate shaped leaves, the only member of the pongamia genus. Micropropagation of several japanese woody plants for. Sprouting of buds was 64% on medium devoid of plant growth regulators pgr. This stage begins with the excision of meristem tissue from an. Detail study on the properties of pongamia pinnata. Technology and application and a great selection of related books, art and collectibles available now at. Tissue culture serves as an alternative to vegetative propagation by conventional methods, such as by rooting of cuttings. Pappilonaceae is a nonedible oil of indian origin lakshmikanthan, 1978. Pongamia pinnata is drought resistant, semideciduous, nitrogen fixing leguminous tree. List of various diseases cured by pongamia pinnata.